Destabilization of medial meniscus

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August undefined, 2024

WebApr 14, 2024 · The meniscus plays a central load‐bearing role in the knee joint. Unfortunately, meniscus injury is common and can lead to joint degeneration and osteoarthritis (OA). In small animal models, progressive degenerative changes occur with the unloading of the meniscus via destabilization of the medial meniscus (DMM). WebApr 7, 2024 · 按文献方法采用内侧半月板失稳(destabilization of medial meniscus，DMM)手术诱导建立KOA模型[9]，简述如下：右膝关节内侧髌韧带与内侧副韧带之间开0.5 cm切口，显微镜下暴露、剪断内侧半月板胫骨韧带，缝合切口。

Osteoarthritis Mouse Model of Destabilization of the Medial …

WebJun 28, 2024 · A mouse OA model was induced using destabilization of the medial meniscus (DMM) surgery, chondrocyte ferroptosis was induced using an intra-articular injection of Erastin, and Met (200 mg/kg/day) was intragastrically administered for 8 weeks after surgery. H&E and Safranin O‑fast green staining were used to evaluate cartilage … WebApr 1, 2024 · Methods Destabilization of the medial meniscus (DMM) or sham surgeries were performed on 10-week-old male wild-type (WT) and Camkk2-/- mice. Half of the DMM-WT mice and all other cohorts ... ipv6 header wiki

The surgical destabilization of the medial meniscus (DMM

WebAnimals (mice or rats) are anesthetized with Isoflurane and the right knee area and lower leg are prepared for surgery. For the surgical destabilization of the medial meniscus (DMM), a skin incision is made from the distal patella to the proximal tibial plateau. The joint capsule immediately medial to the patellar tendon is incised and spread ... WebApr 10, 2024 · Methods: In the present study, C57BL/6J mice were divided into the following three groups: the sham control, destabilization of the medial meniscus (DMM), and AMD3100-treated DMM (DMM + AMD3100) groups. The mice were sacrificed after 2 or 8 weeks, and samples were collected for histological and immunohistochemical analyses. … orchestra with typewriter

Kindlin-2 preserves integrity of the articular cartilage to protect ...

Gender Differences in Mice after Medial Meniscus …

WebFeb 12, 2024 · The DMM (destabilization of the medial meniscus) model is now the most commonly used surgical model in mice. Its reliability, reproducibility, structural similarity to human OA, its relatively... WebMay 1, 2024 · Conclusions. We developed medial meniscus extrusion in a rat model, and centralization of the extruded medial meniscus by the pull-out suture technique improved the medial meniscus extrusion and delayed cartilage degeneration, though the effect was limited. Centralization is a promising treatment to prevent the progression of osteoarthritis. ipv6 hierarchical addressingWebJan 1, 2015 · Destabilization of the medial meniscus (DMM) surgery and tissue processing post-DMM. DMM surgeries were performed, as described 32, 33 , in 12-week-old male IKKα cKO and WT littermates. Power... ipv6 headers flow label

"WebDestabilization of medial meniscus (DMM) model is an important tool for studying the pathophysiological roles of numerous arthritis associated molecules in the pathogenesis of osteoarthritis (OA) in vivo. However, the detailed, especially the visualized protocol for establishing this complicated model in mice, is not available. " - Destabilization of medial meniscus

Destabilization of medial meniscus

Age-dependent Changes in the Articular Cartilage and …

WebJul 6, 2024 · Destabilization Medial Meniscus Cartilage Scratch Murine Model. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch … WebThe surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA).

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WebInduction of DMM: Animals (mice or rats) are anesthetized with Isoflurane and the right knee area and lower leg are prepared for surgery. For the surgical destabilization of the medial meniscus (DMM), a skin incision … WebMar 29, 2024 · When the destabilization of the medial meniscus (DMM) osteoarthritis model was induced in RUNX2 knockout mice, the gene expression of matrix degradation …

WebSep 1, 2007 · A: Surgical approach to the right mouse knee joint with a medial para-patellar ligament incision; B: Following fat pad blunt dissection the medial meniscus (MM) and … WebIn the µCT analysis, destruction of the medial tibial plateau was observed in 4/40 knees, while none were observed in the DMM group. Of the four knees, destruction of the …

WebApr 10, 2024 · In this study, a destabilization of the medial meniscus (DMM)-induced OA model was constructed, and SDF-1α expression in the OA model was evaluated. The CXCR4 receptor antagonist AMD3100 was used to block SDF-1α, and changes in subchondral bone and the expression of BMSCs in subchondral bone in the OA model … WebSep 29, 2016 · Then we induced OA in rats through surgical destabilization of the medial meniscus (DMM), and evaluated the effects of AMD3100 on the progression of OA and aggrecan metabolism. Third, we cultured rat primary chondrocytes with untreated control, CXCL12a, siNC + CXCL12a, or siRNA CXCR4 + CXCL12a and confirmed ACAN, SOX …

WebFeb 10, 2024 · We further investigated whether kindlin-2 loss impacts progression of a destabilization of the medial meniscus (DMM)-induced OA model. At 2 months of age, the K2fl/fl; AggrecanCreERT2 mice were...

Webdifferences in a destabilized medial meniscus osteoarthritis model. Methods: Male and Female mice receive either a destabilized medial meniscus or sham procedure resulting in groups of n=5 (n=20 total). The mice were then allowed to explore a specialized gait arena to collect spatiotemporal data. Gait results were analyzed for gender differences. orchestra viewWebFeb 9, 2024 · Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating … ipv6 history timelineWebNov 11, 2024 · Mice subjected to surgery for destabilization of the medial meniscus (OA group) or control surgery (Control group) were sacrificed after eight weeks. The knees were collected for histological assessment. orchestra vente flashWebFeb 9, 2024 · Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating … orchestra view kennedy centerWebFeb 8, 2024 · This chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also … orchestraded sw architecturehttp://jur.byu.edu/?p=1626 ipv6 home agentWebThis chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also provided, as well as details on osteoarthritis evaluation. Related articles Based on techniques Generation and Characterization of Mouse Models for Skeletal Disease ipv6 history