WebFeb 18, 2024 · After washed five times with phosphate-buffered solution (PBS, Hyclone, Logan, UT, USA) and cut into small pieces about 1 mm 2 within 2 h, the tissues were seeded at the bottom of 25 cm 2 cell culture flask (Corning, NY, USA) and cultured in DMEM with 20% fetal bovine serum (FBS, BioInd, Kibbutz, Israel) and 1% antibiotics at 37 °C in … WebDec 1, 2024 · Prior to transfection, HEK293T cells were cultured at 37 °C in DMEM (SparkJade, China) supplemented with 10% fetal bovine serum (FBS) (BioInd, Israel). 24 h before transfection, cells were seeded into 24-well plates at the concentration of 1 × 10 5 per well. Then, 500 ng of TNFα promoter construct of different lengths in pGL3-basic, 300 ng ...
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WebSerum is commonly used as a supplement to basal growth medium in cell culture. The most common type of serum used for cell growth is foetal bovine serum (FBS), also known as foetal calf serum (FCS). Foetal bovine serum is obtained from foetuses harvested in abattoirs from healthy dams fit for human consumption. WebBIOND BIOLOGICS Innovative therapies for novel oncology targets BIOND BIOLOGICS Enabling the intracellular delivery of biologics how do llamas communicate
Exosomes from adipose-derived stem cells regulate M1/M2 …
WebDec 20, 2024 · Gonad tissues were collected from healthy M. albus specimens, and the tissues were washed with cold foetal bovine serum (FBS), ... (Thermo Fisher Scientific) containing 10% FBS (BioInd) and 1% P/S (Thermo Fisher Scientific) for 24 h before transfection. The cells were seeded onto the 24-well plates at a concentration of 1 × 10 5 … WebJun 5, 2024 · All cells were cultured in DMEM (Hyclone, Logan, UT, USA) containing 10% foetal bovine serum (FBS) (BioInd, Kibbutz, Israel) at 37 °C with 5% CO 2. When reached 80% confluence, cells were scraped, dissociated and counted and then plated in 6-well plates at a concentration of 2 × 10 5 /ml (in RAW264.7 cells) or 2 × 10 6/ ml(in BMDMs … WebAug 12, 2024 · Cell culture. Animal protocol was approved by Zhejiang University according to the Chinese Guidelines for Laboratory Animal Care and Use. Bone marrow was isolated from femurs and tibias of 8-week-old C57BL/6J male mice, and BM-MSCs were obtained as described previously [] and cultured in DMEM medium (Hyclone, USA) supplemented … how much potato salad for 12 people